PathScan®RP ZO-1 Sandwich ELISA Kit#94695,Cell Signaling Technology (CST),94695
The PathScan®RP ZO-1 Sandwich ELISA Kit detects endogenous levels of ZO-1 protein. This kit sensitivity is shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Reactivity
Human, Monkey
Platform ID
BAB523072007
Cell Signaling Technology (CST)
Contact
Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355
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Specifications
Scientific Background
Tight junctions, or zona occludens (ZO), form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces (reviewed in 1). ZO-1, -2, and -3 (also known as TJP1, 2, and 3) are peripheral membrane adaptor proteins that link junctional transmembrane proteins, such as occludin and claudin, to the actin cytoskeleton (reviewed in 2). ZO-1 and ZO-2 are required for tight junction formation and function (3,4). In subconfluent proliferating cells, ZO-1 and ZO-2 have been shown to colocalize to the nucleus and play a role in transcriptional regulation, possibly through facilitating nuclear import/export of transcriptional regulators (5-7). TheZO-2gene is transcribed from two promoters, generating the ZO-2A and ZO-2C isoforms. ZO-2C lacks a 23 amino acid amino-terminal sequence found in other ZO-2 isoforms. While both isoforms appear to be widely expressed, abnormal regulation of theZO-2gene may be correlated with development of ductal cancer (8).Shin, K. et al. (2006)Annu Rev Cell Dev Biol22, 207-35.Matter, K. and Balda, M.S. (2007)J Cell Sci120, 1505-11.Hernandez, S. et al. (2007)Exp Cell Res313, 1533-47.Umeda, K. et al. (2006)Cell126, 741-54.Betanzos, A. et al. (2004)Exp Cell Res292, 51-66.Traweger, A. et al. (2003)J Biol Chem278, 2692-700.Huerta, M. et al. (2007)Mol Biol Cell18, 4826-36.Chlenski, A. et al. (2000)Biochim Biophys Acta1493, 319-24.Alternate NamesDKFZp686M05161; MGC133289; Tight junction protein 1; tight junction protein 1 (zona occludens 1); Tight junction protein ZO-1; TJP1; ZO-1; ZO1; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1
Synonyms
DKFZp686M05161; MGC133289; Tight junction protein 1; tight junction protein 1 (zona occludens 1); Tight junction protein ZO-1; TJP1; ZO-1; ZO1; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1
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