PerCP/Cyanine5.5 anti-H2A.X-Phosphorylated Ser139 Antibody anti-Phosphorylated H2A.X - 2F3,BioLegend,613413

Additional reported applications (for the relevant formats of this clone) include:immunohistochemistry on paraffin embedded sections2, immunofluorescence microscopy3-9, Western blotting10-12, and flow cytometry1,13. Clone 2F3 cross-reacts with mouse4.Intracellular staining protocol for Anti-H2A.X-Phosphorylated (Ser139) Antibodyfor Flow Cytometry1. Prepare 70% absolute ethanol. Chill solution by storing at -20°C.2. Prepare cells of interest.3. Wash 1X with PBS, centrifuge at 350g for 5 min.4. Discard the supernatant and vortex to loosen cell pellet.5. Add pre-cooled 70% ethanol drop by drop, while vortexing.6. Incubate at -20°C for 60 minutes.7. Wash 3X withBioLegend Cell Staining Bufferand resuspend the cells at 0.5-1 X 107cells/ml in the cell staining buffer.8. Perform immunofluorescent staining for flow cytometry.

Host

Mouse

Reactivity

Human, Mouse

Application

ICFC - Quality tested

Platform ID

BAB490532691

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

Email:

Product Specifications
Scientific Background

Specifications

NamePerCP/Cyanine5.5 anti-H2A.X-Phosphorylated Ser139 Antibody anti-Phosphorylated H2A.X - 2F3
Cat. No.613413
HostMouse
RRIDAB_2616872 (BioLegend Cat. No. 613413)AB_2616873 (BioLegend Cat. No. 613414)
IsotypeMouse IgG1, κ
ReactivityHuman, Mouse
ApplicationICFC - Quality tested
ClonalityMonoclonal
Clone Number2F3
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetH2A.X Phospho Ser139
ImmunogenModified peptide
PurityThe antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

H2A.X is a 14 kD basal histone and a member of the H2 histone family. This nuclear protein is synthesized in the G1 and S phase of the cell cycle and is known to be important for DNA repair and maintaining genomic stability and for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 after double-stranded DNA breaks. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability. The 2F3 monoclonal antibody reacts with phosphorylated human H2A.X (Ser139) and has been shown to be useful for Western blotting, immunofluorescence and flow cytometry.

Category Paths

Request a product

Please provide the required information below so that we can quickly source your products.