PerCP/Cyanine5.5 anti-RPS6 Phospho (Ser244) Antibody, RPS6 Phospho, A18024A,BioLegend,935710

BioLegend offers two clones against this target, A18024A and A18024B:For western blotting, A18024B displayed a higher affinity for RPS6 Phospho (Ser244) compared to A18024A. Both clones exhibit mouse reactivity.For ICC, A18024B displayed a moderately higher affinity for RPS6 Phospho (Ser244) compared to A18024A. Both clones are mouse reactive for this application, and both clones are compatible with Triton X-100 and methanol permeabilization steps.For ICFC, A18024A works in all three ICFC buffers (Cat# 425401, 421002, 424401). For ICFC, A18024A weakly stains mouse RPS6 Phospho (Ser244). A18024B is not recommended for ICFC due to high background staining.Both clones are predicted to react with rat RPS6 when phosphorylated at serine 244 due to complete sequence homology between the immunizing sequence and the rat RPS6 ortholog.

Host

Mouse

Reactivity

Human, Mouse

Application

ICFC - Quality tested

Platform ID

BAB801017380

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Product Specifications
Scientific Background

Specifications

NamePerCP/Cyanine5.5 anti-RPS6 Phospho (Ser244) Antibody, RPS6 Phospho, A18024A
Cat. No.935710
HostMouse
RRIDAB_2892496 (BioLegend Cat. No. 935709)AB_2892496 (BioLegend Cat. No. 935710)
IsotypeMouse IgG1, κ
ReactivityHuman, Mouse
ApplicationICFC - Quality tested
ClonalityMonoclonal
Clone NumberA18024A
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetRPS6 Phospho
ImmunogenSynthetic peptide corresponding to human RPS6 phosphorylated at serine 244
PurityThe antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

Ribosomal protein S6 (RPS6) is a key component of the small 40S ribosomal subunit and is the major substrate of protein kinases in eukaryotic ribosomes. In response to various cellular stimuli such as mitogenic stimulation, insulin, and increased nutrient availability, upstream kinases such as RSK and p70 kinases phosphorylate RPS6 at multiple serine sites. These modifications facilitate the recruitment of the 7-methylguanasine cap complex, thereby promoting the assembly of the translational pre-initiation complex and increased cellular protein synthesis capacity. RPS6 has been shown to be hyperphosphorylated in certain cancers, and phosphorylation is a critical determinant of pancreatic β–cell size and systemic glucose homeostasis function in diabetic mouse models.

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