PerCP/Cyanine5.5 anti-Vimentin,BioLegend,677814

While this clone recognizes mouse Vimentin, we do not recommend its usage for western blot due to poor affinity of the antibody for the protein. Additional reported applications for the relevant formats include: spatial biology (IBEX)1,2.

Host

Mouse

Reactivity

Human

Application

ICFC - Quality testedSB - Community Verified

Platform ID

BAB199005105

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

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Contact

Tel: 1-858-455-9588
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Product Specifications
Scientific Background

Specifications

NamePerCP/Cyanine5.5 anti-Vimentin
Cat. No.677814
HostMouse
RRIDAB_3106228 (BioLegend Cat. No. 677813)AB_3106228 (BioLegend Cat. No. 677814)
IsotypeMouse IgG2a, κ
ReactivityHuman
ApplicationICFC - Quality testedSB - Community Verified
ClonalityMonoclonal
Clone NumberO91D3
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetVimentin
ImmunogenFull length human vimentin produced inE. coli.
PurityThe antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

Vimentin are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is a widely expressed and highly conserved 54 kD protein that is constitutively expressed in mesenchymal cells, endothelial cells lining blood vessels, renal tubular cells, macrophages, neutrophils, fibroblasts, and leukocytes1,2. Vimentin is used as a marker of mesenchymal cells to distinguish them from epithelial cells3. Increased vimentin expression is frequently used as an EMT marker in cancer4. Autoantibodies to vimentin are commonly found in patients with autoimmune diseases such as Lupus5and rheumatoid arthritis6, and also found after transplantation7.

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