PerCP/Cyanine5.5 anti-mouse CD25 Antibody anti-CD25 - PC61,BioLegend,102030
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2,in vitroblocking of IL-2 binding to low- and high-affinity receptors1-4, growth inhibition of IL-2-dependent T-cell lines1-4,in vivodepletion of CD25+CD4+Treg cells5-8,10, and immunohistochemical staining of acetone-fixed frozen sections2. PC61 antibody recognizes a different epitope than 3C7 antibody (Cat. No. 101902). Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 102040) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered.
Host
Rat
Reactivity
Mouse
Application
FC - Quality tested
Platform ID
BAB766134132
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
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Product Specifications
Scientific Background
Specifications
NamePerCP/Cyanine5.5 anti-mouse CD25 Antibody anti-CD25 - PC61
Cat. No.102030
HostRat
RRIDAB_893291 (BioLegend Cat. No. 102029)AB_893288 (BioLegend Cat. No. 102030)
IsotypeRat IgG1, λ
ReactivityMouse
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone NumberPC61
Concentration0.2 mg/ml
TargetCD25
ImmunogenIL-2-dependent cytolytic mouse T-cell clone B6.1
PurityThe antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only
Scientific Background
CD25 is a 55 kD glycoprotein also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor.
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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