PerCP/Cyanine5.5 anti-mouse H-2KbH-2Db Antibody anti-H-2KbH-2Db - 28-8-6,BioLegend,114620

Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity1, and immunohistochemical staining2of acetone-fixed frozen sections.

Host

Mouse

Reactivity

Mouse

Application

FC -Quality tested

Platform ID

BAB236309719

BioLegend

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Contact

Tel: 1-858-455-9588
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Product Specifications
Scientific Background

Specifications

NamePerCP/Cyanine5.5 anti-mouse H-2KbH-2Db Antibody anti-H-2KbH-2Db - 28-8-6
Cat. No.114620
HostMouse
RRIDAB_2750199 (BioLegend Cat. No. 114619)AB_2750200 (BioLegend Cat. No. 114620)
IsotypeMouse (C3H) IgG2a, κ
ReactivityMouse
ApplicationFC -Quality tested
ClonalityMonoclonal
Clone Number46971
Concentration0.2 mg/ml
TargetH-2Kb/H-2Db
ImmunogenC3H.SW mouse splenocytes
PurityThe antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

The 28-8-6 antibody reacts with the H-2Kband H-2DbMHC class I alloantigens expressed on nucleated cells from mice of the H-2Kb/H-2Dbhaplotype. H-2Kb/H-2Dbis involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 28-8-6 antibody cross-reacts with H-2DdMHC class I alloantigen, but does not react with alloantigens of f, k, p, q, r, s haplotypes.

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