PerCP/Cyanine5.5 anti-mouse IL-22 Antibody anti-IL-22 - Poly5164,BioLegend,516411

ELISA Capture:The purified Poly5164 antibody is useful as a capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5164 antibody (Cat. No. 516407) as the detecting antibody and recombinant mouse IL-22 (Cat. No. 576209) as the standard.In sandwich ELISA, Poly5164 has approximately 17% and 2% cross-reactivity with recombinant rat and human IL-22, respectively.Flow Cytometry:The fluorochrome-labeled Poly5164 antibody is useful for immunofluorescent staining and flow cytometric analysis to identify IL-22-producing cells within mixed cell populations.Note:For testing mouse IL-22 in serum, plasma or supernatant, LEGEND MAX™ Mouse IL-22 ELISA Kit (Cat. No. 436307 & 436308) is specially developed and recommended.Note:The use of Iscove's Modified Dulbecco's Medium (IMDM) will result in betterin vitroTh17 polarization.1

Host

Goat

Reactivity

Mouse

Application

ICFC -Quality tested

Platform ID

BAB936674762

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Product Specifications
Scientific Background

Specifications

NamePerCP/Cyanine5.5 anti-mouse IL-22 Antibody anti-IL-22 - Poly5164
Cat. No.516411
HostGoat
RRIDAB_2563373 (BioLegend Cat. No. 516411)
IsotypeGoat Polyclonal IgG
ReactivityMouse
ApplicationICFC -Quality tested
ClonalityPolyclonal
Clone NumberPoly5164
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetIL-22
ImmunogenMouse IL-22, amino acids Leu34-Val179 (Accession# NM_016971), was expressed inE. coli.
PurityThe antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

IL-22 is a cytokine structurally related to IL-10.  Mouse IL-22 consists of 179 amino acids and has a predicted molecular weight of 20 kD.  It is secreted primarily by Th17, Th1, Th2, lymphoid tissue inducer cells (LTi), and subsets of natural killer cells.  It has been reported that aryl hydrocarbon receptor (AhR) expression is essential for the production of IL-22 by TCRγδ T cells.  AhR activation increases Th17 polarization and induces IL-22 production.  IL-22 functions by engaging the heterodimeric IL-22 receptor (IL-22R) complex, consisting of two receptor subunits, IL-22R1 and IL-10Rβ.  IL-22 acts on nonhematopoietic tissue cells, such as epithelial cells of the digestive and respiratory systems and keratinocytes of the skin.  IL-22 is involved in inflammatory processes such as dermal inflammation, psoriasis, inflammatory bowel disease, hepatitis, and Crohn’s disease.  Moreover, it plays a critical role in mucosal immunity and the wound healing process.

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