PerCP/Fire™ 780 anti-human CD45RO Antibody, CD45RO, UCHL1,BioLegend,304278

The UCHL1 antibody is commonly used in combination with antibodies against CD45RA to discern memory and naïve T cells. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5and formalin-fixed paraffin-embedded tissue sections4, Western blotting2, and immunoprecipitation3.UCHL1 signal intensity may be reduced in the presence of True-Stain Monocyte Blocker™ (Cat. No. 426102). Clone S19021B is not impacted by Monocyte Blocker and is recommended as an alternative to UCHL1.

Host

Mouse

Reactivity

Human

Application

FC - Quality tested

Platform ID

BAB098830683

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Contact

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Product Specifications
Scientific Background

Specifications

NamePerCP/Fire™ 780 anti-human CD45RO Antibody, CD45RO, UCHL1
Cat. No.304278
HostMouse
RRIDAB_3675031 (BioLegend Cat. No. 304277)AB_3675031 (BioLegend Cat. No. 304278)
IsotypeMouse IgG2a, κ
ReactivityHuman
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone NumberUCHL1
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetCD45RO
ImmunogenIL-2 dependent T cell line, CA1
PurityThe antibody was purified by affinity chromatography and conjugated with PerCP/Fire™ 780 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD45RO is a 180 kD single chain membrane glycoprotein. It is a splice variant of tyrosine phosphatase CD45, lacking the A, B, and C determinants. The CD45RO isoform is expressed on activated and memory T cells, some B cell subsets, activated monocytes/macrophages, and granulocytes. CD45RO enhances both T cell receptor and B cell receptor signaling mediated activation. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1 and CD22. CD45 isoform expression can change in response to cytokines.

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