Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit Monoclonal Antibody (SignalFlexTMAlexa Fluor®532 Conjugate)#57330,Cell Signaling Technology (CST),57330

Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (SignalFlexTM Alexa Fluor®532 Conjugate) detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. Non-specific staining has been observed in mitotic cells by immunofluorescence.

Host

Rabbit

Reactivity

Human, Mouse, Rat, Monkey, D. melanogaster

Conjugate

SignalFlexTMAlexa Fluor®532 Conjugate

Platform ID

BAB389213761

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NamePhospho-4E-BP1 (Thr37/46) (236B4) Rabbit Monoclonal Antibody (SignalFlexTMAlexa Fluor®532 Conjugate)#57330
Cat. No.57330
Accession NumberQ13541
Gene ID (Entrez)13541, 1978
HostRabbit
SensitivityEndogenous
ReactivityHuman, Mouse, Rat, Monkey, D. melanogaster
ConjugationSignalFlexTMAlexa Fluor®532 Conjugate
ImmunogenIgG
FormulationSignalFlexTM conjugates are produced using highly validated Cell Signaling Technology®primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays.Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.
StorageSupplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C.Do not aliquot the antibody. Protect from light. Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylatedin vivo(4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).Pause, A. et al. (1994)Nature371, 762-7.Brunn, G.J. et al. (1997)Science277, 99-101.Gingras, A.C. et al. (1998)Genes Dev12, 502-13.Fadden, P. et al. (1997)J Biol Chem272, 10240-7.Gingras, A.C. et al. (1999)Genes Dev13, 1422-37.Alternate Names4E-BP1; 4EBP1; BP-1; eIF4E-binding protein 1; EIF4EBP1; eukaryotic translation initiation factor 4E binding protein 1; Eukaryotic translation initiation factor 4E-binding protein 1; MGC4316; PHAS-I; Phosphorylated heat- and acid-stable protein regulated by insulin 1

Synonyms

4E-BP1; 4EBP1; BP-1; eIF4E-binding protein 1; EIF4EBP1; eukaryotic translation initiation factor 4E binding protein 1; Eukaryotic translation initiation factor 4E-binding protein 1; MGC4316; PHAS-I; Phosphorylated heat- and acid-stable protein regulated by insulin 1

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