Phospho-ATM/ATR Substrate Motif [(pS/pT) QG] MultiMab®Rabbit Monoclonal Antibody mix#6966,Cell Signaling Technology (CST),6966

Phospho-ATM/ATR Substrate Motif [(pS/pT) QG] MultiMab®Rabbit Monoclonal Antibody mix recognizes proteins containing phospho-Ser or phospho-Thr followed by Gln and Gly residues. To some extent, this antibody also recongizes proteins with an S*/T*Q motif.

Host

Rabbit

Reactivity

All Species Expected

Application

Western Blotting: 1:1000 Immunoprecipitation: 1:100

Platform ID

BAB119886778

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background

Specifications

NamePhospho-ATM/ATR Substrate Motif [(pS/pT) QG] MultiMab®Rabbit Monoclonal Antibody mix#6966
Cat. No.6966
HostRabbit
SensitivityEndogenous
ReactivityAll Species Expected
ApplicationWestern Blotting: 1:1000 Immunoprecipitation: 1:100
ImmunogenIgG
FormulationSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
StorageSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Regulatory StatusResearch Use Only

Scientific Background

Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair (1). The identified substrates for ATM are p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1, and Chk1 (1,2) The essential requirement for the substrates of ATM/ATR is S*/T*Q. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate recognition by these kinases. Positively charged residues surrounding the S*/T*Q are negative determinants for substrate phosphorylation (3). The complex phenotype of AT cells suggests that it likely has additional substrates (3). To better understand the kinase and identify substrates for ATM and the related kinase ATR, CST has developed antibodies that recognize phosphorylated serine or threonine in the S*/T*Q motif.Kastan, M.B. and Lim, D.S. (2000)Nature Rev. Mol. Cell Biol.1, 179-186.Zhao, H. and Piwnica-Worms, H. (2001)Mol. Cell. Biol.21, 4129-4139.Kim, S. T. et al. (1999)J. Biol. Chem.274, 37538-37543.

Category Paths

Request a product

Please provide the required information below so that we can quickly source your products.