Phospho-Bcl-2 (Ser70) (5H2) Rabbit Monoclonal Antibody (SignalFlexTMPacific BlueTMConjugate)#66915,Cell Signaling Technology (CST),66915

Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb (SignalFlexTM Pacific BlueTM Conjugate) detects endogenous levels of Bcl-2 only when phosphorylated at serine 70. The antibody does not cross-react with nonphosphorylated Bcl-2 at endogenous levels or with other Bcl-2 family members.

Host

Rabbit

Reactivity

Human

Conjugate

SignalFlexTMPacific BlueTMConjugate

Platform ID

BAB264551099

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NamePhospho-Bcl-2 (Ser70) (5H2) Rabbit Monoclonal Antibody (SignalFlexTMPacific BlueTMConjugate)#66915
Cat. No.66915
Accession NumberP10415
Gene ID (Entrez)10415
HostRabbit
SensitivityEndogenous
ReactivityHuman
ConjugationSignalFlexTMPacific BlueTMConjugate
ImmunogenIgG
FormulationSignalFlexTM conjugates are produced using highly validated Cell Signaling Technology®primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays.Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.
StorageSupplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C.Do not aliquot the antibody. Protect from light. Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

Bcl-2 exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release (1). It has been implicated in modulating mitochondrial calcium homeostasis and proton flux (2). Several phosphorylation sites have been identified within Bcl-2, including Thr56, Ser70, Thr74, and Ser87 (3). It has been suggested that these phosphorylation sites may be targets of the ASK1/MKK7/JNK1 pathway and that phosphorylation of Bcl-2 may be a marker for mitotic events (4,5). Mutation of Bcl-2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes (6). Interleukin-3 and JNK-induced Bcl-2 phosphorylation at Ser70 may be required for its enhanced anti-apoptotic functions (7).Murphy, K.M. et al. (2000)Cell Death Differ7, 102-11.Zhu, L. et al. (1999)J Biol Chem274, 33267-73.Maundrell, K. et al. (1997)J Biol Chem272, 25238-42.Yamamoto, K. et al. (1999)Mol Cell Biol19, 8469-78.Ling, Y.H. et al. (1998)J Biol Chem273, 18984-91.Huang, S.T. and Cidlowski, J.A. (2002)FASEB J16, 825-32.Deng, X. et al. (2001)J Biol Chem276, 23681-8.Alternate NamesApoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; Bcl-2; BCL2; BCL2 apoptosis regulator; PPP1R50; protein phosphatase 1, regulatory subunit 50

Synonyms

Apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; Bcl-2; BCL2; BCL2 apoptosis regulator; PPP1R50; protein phosphatase 1, regulatory subunit 50

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