Phospho-CREB (Ser133) (87G3) Rabbit Monoclonal Antibody (SignalFlexTMAlexa Fluor®750 Conjugate)#84059,Cell Signaling Technology (CST),84059

Phospho-CREB (Ser133) (87G3) Rabbit mAb (SignalFlexTM Alexa Fluor®750 Conjugate) detects endogenous levels of CREB only when phosphorylated at serine 133. The antibody also detects the phosphorylated form of the CREB-related protein, ATF-1.

Host

Rabbit

Reactivity

Human, Mouse, Rat

Conjugate

SignalFlexTMAlexa Fluor®750 Conjugate

Platform ID

BAB702419672

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NamePhospho-CREB (Ser133) (87G3) Rabbit Monoclonal Antibody (SignalFlexTMAlexa Fluor®750 Conjugate)#84059
Cat. No.84059
Accession NumberP16220
Gene ID (Entrez)16220, 1385
HostRabbit
SensitivityEndogenous
ReactivityHuman, Mouse, Rat
ConjugationSignalFlexTMAlexa Fluor®750 Conjugate
ImmunogenIgG
FormulationSignalFlexTM conjugates are produced using highly validated Cell Signaling Technology®primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays.Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.
StorageSupplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C.Do not aliquot the antibody. Protect from light. Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways, including Erk, Ca2+, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9).Lonze, B.E. et al. (2002)Neuron34, 371-85.Lee, M.M. et al. (1999)J Neurosci Res55, 702-12.Redmond, L. et al. (2002)Neuron34, 999-1010.Dash, P.K. et al. (1990)Nature345, 718-21.Yin, J.C. et al. (1994)Cell79, 49-58.Guzowski, J.F. and McGaugh, J.L. (1997)Proc Natl Acad Sci USA94, 2693-8.Xing, J. et al. (1998)Mol Cell Biol18, 1946-55.Ribar, T.J. et al. (2000)J Neurosci20, RC107.Tan, Y. et al. (1996)EMBO J15, 4629-42.Alternate Namesactive transcription factor CREB; cAMP responsive element binding protein 1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CREB; CREB-1; CREB1; cyclic adenosine 3',5'-monophosphate response element binding protein; cyclic adenosine 3',5'-monophosphate response element-binding protein CREB; Cyclic AMP-responsive element-binding protein 1; MGC9284; transactivator protein

Synonyms

active transcription factor CREB; cAMP responsive element binding protein 1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CREB; CREB-1; CREB1; cyclic adenosine 3',5'-monophosphate response element binding protein; cyclic adenosine 3',5'-monophosphate response element-binding protein CREB; Cyclic AMP-responsive element-binding protein 1; MGC9284; transactivator protein

Category Paths

Request a product

Please provide the required information below so that we can quickly source your products.