Phospho-HER2/ErbB2 (Tyr1196) (D66B7) Rabbit Monoclonal Antibody#6942,Cell Signaling Technology (CST),6942

The ErbB2 (HER2) proto-oncogene encodes a 185 kDa transmembrane, receptor-like glycoprotein with intrinsic tyrosine kinase activity (1). While ErbB2 lacks an identified ligand, ErbB2 kinase activity can be activated in the absence of a ligand when overexpressed and through heteromeric associations with other ErbB family members (2). Amplification of theErbB2gene and overexpression of its product are detected in almost 40% of human breast cancers (3). Binding of the c-Cbl ubiquitin ligase to ErbB2 at Tyr1112 leads to ErbB2 poly-ubiquitination and enhances degradation of this kinase (4). ErbB2 is a key therapeutic target in the treatment of breast cancer and other carcinomas and targeting the regulation of ErbB2 degradation by the c-Cbl-regulated proteolytic pathway is one potential therapeutic strategy. Phosphorylation of the kinase domain residue Tyr877 of ErbB2 (homologous to Tyr416 of pp60c-Src) may be involved in regulating ErbB2 biological activity. The major autophosphorylation sites in ErbB2 are Tyr1248 and Tyr1221/1222; phosphorylation of these sites couples ErbB2 to the Ras-Raf-MAP kinase signal transduction pathway (1,5).The autophosphorylation of HER/ErbB2 at Tyr1196 mediates HER2/ErbB2 association with Crk protein and leads to Ras-independent downstream Erk acitvation (6). HER2/ErbB2 phosphorylation at Tyr1196 has been coupled to cell migration and polarity disruption (7,8).Muthuswamy, S.K. et al. (1999)Mol Cell Biol19, 6845-57.Qian, X. et al. (1994)Proc Natl Acad Sci USA91, 1500-4.Dittadi, R. and Gion, M. (2000)J Natl Cancer Inst92, 1443-4.Klapper, L.N. et al. (2000)Cancer Res60, 3384-8.Kwon, Y.K. et al. (1997)J Neurosci17, 8293-9.Dankort, D. et al. (2001)Mol Cell Biol21, 1540-51.Marone, R. et al. (2004)Nat Cell Biol6, 515-22.Lucs, A.V. et al. (2010)Oncogene29, 174-87.Alternate Namesc-erb B2/neu protein; CD340; erb-b2 receptor tyrosine kinase 2; erbB-2; ERBB2; HER-2; HER-2/neu; HER2; herstatin; human epidermal growth factor receptor 2; Metastatic lymph node gene 19 protein; MLN 19; MLN19; NEU; neuro/glioblastoma derived oncogene homolog; neuroblastoma/glioblastoma derived oncogene homolog; NGL; p185erbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; Receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog); v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

c-erb B2/neu protein; CD340; erb-b2 receptor tyrosine kinase 2; erbB-2; ERBB2; HER-2; HER-2/neu; HER2; herstatin; human epidermal growth factor receptor 2; Metastatic lymph node gene 19 protein; MLN 19; MLN19; NEU; neuro/glioblastoma derived oncogene homolog; neuroblastoma/glioblastoma derived oncogene homolog; NGL; p185erbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; Receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog); v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)