Phospho-HS1 (Tyr378/397) (D12C1) Rabbit Monoclonal Antibody (SignalFlex^TMAlexa Fluor^®700 Conjugate)#92975,Cell Signaling Technology (CST),92975

HS1 (HCLS1, LckBP1, p75) is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (1,2). HS1 contains four cortactin repeats and a single SH3 domain (2). This intracellular protein is phosphorylated following immune receptor activation, which promotes recruitment of HS1 to the immune synapse (3-5). Phosphorylation of HS1 is required to regulate actin dynamics and provide docking sites for many other signaling molecules, such as Vav1 and PLCγ1 (6). HS1 also plays an important role in platelet activation (7).HS1 is rapidly phosphorylated at Tyr397 by Syk and/or Lyn kinases following immune receptor stimulation and thrombin-mediated platelet stimulation. This phosphorylation is an important step in cytoskeletal rearrangement and signaling complex formation (6-10).Kitamura, D. et al. (1989)Nucleic Acids Res17, 9367-79.Kitamura, D. et al. (1995)Biochem Biophys Res Commun208, 1137-46.Suzuki, H. et al. (1997)J Immunol159, 5881-8.Hata, D. et al. (1994)Immunol Lett40, 65-71.Yamanashi, Y. et al. (1993)Proc Natl Acad Sci USA90, 3631-5.Gomez, T.S. et al. (2006)Immunity24, 741-52.Kahner, B.N. et al. (2007)Blood110, 2449-56.Yamanashi, Y. et al. (1997)J Exp Med185, 1387-92.Hao, J.J. et al. (2004)J Biol Chem279, 33413-20.Brunati, A.M. et al. (2005)J Biol Chem280, 21029-35.Alternate Namescortactin-like; CTTNL; HCLS1; Hematopoietic cell-specific LYN substrate 1; Hematopoietic lineage cell-specific protein; HS1; LckBP1; p75

cortactin-like; CTTNL; HCLS1; Hematopoietic cell-specific LYN substrate 1; Hematopoietic lineage cell-specific protein; HS1; LckBP1; p75