Phospho-Myosin Light Chain 2 (Thr18/Ser19) (E2J8F) Rabbit Monoclonal Antibody#95777,Cell Signaling Technology (CST),95777

Phospho-Myosin Light Chain 2 (Thr18/Ser19) (E2J8F) Rabbit Monoclonal Antibody recognizes endogenous levels of myosin light chain 2 protein only when phosphorylated at Thr18 and Ser19.

Host

Rabbit

Reactivity

Human, Mouse, Rat

Application

Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:400 - 1:800

Platform ID

BAB123385098

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NamePhospho-Myosin Light Chain 2 (Thr18/Ser19) (E2J8F) Rabbit Monoclonal Antibody#95777
Cat. No.95777
Accession NumberP24844
Gene ID (Entrez)24844, 10398
HostRabbit
SensitivityEndogenous
ReactivityHuman, Mouse, Rat
ApplicationWestern Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:400 - 1:800
Molecular Weight18
ImmunogenIgG
FormulationSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C.Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #72789.
StorageSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C.Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #72789.
Regulatory StatusResearch Use Only

Scientific Background

Myosin is composed of six polypeptide chains: two identical heavy chains and two pairs of light chains. Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC), RLC, or LC20, has many isoforms depending on its distribution. In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+/calmodulin-dependent manner (1). This phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction (2). ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fibers (3). Phosphorylation of smooth muscle MLC2 at Ser1/Ser2 and Ser9 by PKC and cdc2 has been reported to inhibit myosin ATPase activity (4,5). Phosphorylation by cdc2 controls the timing of cytokinesis (5). Transgenic mice lacking phosphorylation sites on the cardiac muscle isoform show morphological and functional abnormalities (6).Ikebe, M. and Hartshorne, D.J. (1985)J. Biol. Chem.260, 10027-10031.Tan, J. L. et al. (1992)Annu. Rev. Biochem.61, 721-759.Totsukawa, G. et al. (2000)J. Cell Biol.150, 797-806.Ikebe, M. et al. (2000)J. Biol. Chem.262, 9569-9573.Satterwhite, L. L. et al. (1992)J. Cell Biol.118, 595-605.Sanbe, A. et al. (1999)J. Biol. Chem.274, 21085-21094.Alternate Names20 kDa myosin light chain; epididymis secretory sperm binding protein; LC20; MGC3505; MLC-2C; MLC2; MRLC1; MYL9; myosin light chain 9; myosin regulatory light chain 1; Myosin regulatory light chain 2, smooth muscle isoform; Myosin regulatory light chain 9; Myosin regulatory light chain MRLC1; Myosin regulatory light polypeptide 9; Myosin RLC; myosin, light chain 9, regulatory; myosin, light polypeptide 9, regulatory; MYRL2

Synonyms

20 kDa myosin light chain; epididymis secretory sperm binding protein; LC20; MGC3505; MLC-2C; MLC2; MRLC1; MYL9; myosin light chain 9; myosin regulatory light chain 1; Myosin regulatory light chain 2, smooth muscle isoform; Myosin regulatory light chain 9; Myosin regulatory light chain MRLC1; Myosin regulatory light polypeptide 9; Myosin RLC; myosin, light chain 9, regulatory; myosin, light polypeptide 9, regulatory; MYRL2

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