Phospho-PKC Substrate Motif [(R/K)XpSX(R/K)] MultiMab®Rabbit Monoclonal Antibody mix#6967,Cell Signaling Technology (CST),6967

Phospho-PKC Substrate Motif [(R/K)XpSX(R/K)] MultiMab®Rabbit Monoclonal Antibody mix detects endogenous levels of many cellular proteins only when phosphorylated at Ser residues surrounded by Arg or Lys at the -2 and +2 positions. This antibody may also cross-react with S*X(R/K) motif-containing proteins. This antibody does not cross-react with nonphosphorylated Ser residues, phospho-Thr in the same motif, or phospho-Ser in other motifs.

Host

Rabbit

Reactivity

Human, All Species Expected

Application

Western Blotting: 1:1000 Immunoprecipitation: 1:50 Flow Cytometry (Fixed/Permeabilized): 1:1600 Peptide ELISA (DELFIA): 1:1000

Platform ID

BAB624334642

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background

Specifications

NamePhospho-PKC Substrate Motif [(R/K)XpSX(R/K)] MultiMab®Rabbit Monoclonal Antibody mix#6967
Cat. No.6967
HostRabbit
SensitivityEndogenous
ReactivityHuman, All Species Expected
ApplicationWestern Blotting: 1:1000 Immunoprecipitation: 1:50 Flow Cytometry (Fixed/Permeabilized): 1:1600 Peptide ELISA (DELFIA): 1:1000
ImmunogenIgG
FormulationSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
StorageSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Regulatory StatusResearch Use Only

Scientific Background

Although protein kinase C (PKC) family members are involved in a number of signal transduction processes including secretion, gene expression, proliferation, and muscle contraction, many PKC substrates continue to be unidentified (1,2). Isozymes of PKC are subdivided into conventional PKCs (cPKC), novel PKCs (nPKC), and atypical PKCs (aPKC). PKCα, βI, βII, and γ isoforms belong to the cPKC group (1). When activated, cPKC isozymes phosphorylate substrates containing Ser or Thr, with Arg or Lys at the -3, -2, and +2 positions, and a hydrophobic amino acid at position +1 (1-3).Nishikawa, K. et al. (1997)J Biol Chem272, 952-60.Pearson, R.B. and Kemp, B.E. (1991)Methods Enzymol200, 62-81.Obata, T. et al. (2000)J Biol Chem275, 36108-15.

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