Phospho-Paxillin (Tyr118) (E9U9F) Rabbit Monoclonal Antibody#69363,Cell Signaling Technology (CST),69363

Phospho-Paxillin (Tyr118) (E9U9F) Rabbit Monoclonal Antibody recognizes endogenous levels of paxillin protein only when phosphorylated at Tyr118.

Host

Rabbit

Reactivity

Human, Mouse, Rat, Monkey

Application

Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:200 - 1:400 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800

Platform ID

BAB686615997

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NamePhospho-Paxillin (Tyr118) (E9U9F) Rabbit Monoclonal Antibody#69363
Cat. No.69363
Accession NumberP49023
Gene ID (Entrez)49023, 5829
HostRabbit
SensitivityEndogenous
ReactivityHuman, Mouse, Rat, Monkey
ApplicationWestern Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:200 - 1:400 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800
Molecular Weight68
ImmunogenIgG
FormulationSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #91115.
StorageSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #91115.
Regulatory StatusResearch Use Only

Scientific Background

Paxillin is a multidomain protein that localizes primarily to focal adhesion sites in the extracellular matrix (1). Paxillin is one of the key components of integrin signaling, and tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by another focal adhesion component, focal adhesion kinase (FAK), at Tyr118 (3,4). Phospho-paxillin (Tyr118) may provide a docking site for recruitment of other signaling molecules to focal adhesions. It has been shown that the SH2 domain of Crk binds to the phosphorylated Tyr118 of paxillin (5).Turner, C.E. (2000)J Cell Sci113 Pt 23, 4139-40.Burridge, K. et al. (1992)J Cell Biol119, 893-903.Bellis, S.L. et al. (1995)J Biol Chem270, 17437-41.Bellis, S.L. et al. (1997)Biochem J325 (Pt 2), 375-81.Schaller, M.D. and Parsons, J.T. (1995)Mol Cell Biol15, 2635-45.Alternate NamesFLJ16691; PAXI; Paxillin; PXN; testicular tissue protein Li 134

Synonyms

FLJ16691; PAXI; Paxillin; PXN; testicular tissue protein Li 134

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