Phospho-Rad17 (Ser645) (D5H5) Rabbit Monoclonal Antibody#6981,Cell Signaling Technology (CST),6981

Phospho-Rad17 (Ser645) (D5H5) Rabbit Monoclonal Antibody recognizes endogenous levels of Rad17 protein only when phosphorylated at Ser645.

Host

Rabbit

Reactivity

Human

Application

Western Blotting: 1:1000 Immunoprecipitation: 1:200

Platform ID

BAB937140745

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NamePhospho-Rad17 (Ser645) (D5H5) Rabbit Monoclonal Antibody#6981
Cat. No.6981
Accession NumberO75943
Gene ID (Entrez)75943, 5884
HostRabbit
SensitivityEndogenous
ReactivityHuman
ApplicationWestern Blotting: 1:1000 Immunoprecipitation: 1:200
Molecular Weight80
ImmunogenIgG
FormulationSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
StorageSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Regulatory StatusResearch Use Only

Scientific Background

The human checkpoint protein Rad17 and its fission and budding yeast orthologues (Schizosaccharomyces pombeRad17 andSaccharomyces cerevisiaeRad24, respectively) are involved in the activation of checkpoint signals in response to DNA damage or disruption of DNA synthesis (1-4). Treatment of human cells with genotoxic agents induces ATM/ATR-dependent phosphorylation of Rad17 at Ser635 and Ser645. Rad17 phosphorylation is a critical early event during checkpoint signaling in DNA-damaged cells (5-7).Griffiths, D.J. et al. (1995)EMBO J14, 5812-23.Li, L. et al. (1999)Oncogene18, 1689-99.Bao, S. et al. (1998)Cell Growth Differ9, 961-7.von Deimling, F. et al. (1999)Hum Genet105, 17-27.Bao, S. et al. (2001)Nature411, 969-74.Post, S. et al. (2001)Proc Natl Acad Sci U S A98, 13102-7.Wang, X. et al. (2001)Cancer Res61, 7417-21.Alternate NamesCCYC; cell cycle checkpoint protein (RAD17); Cell cycle checkpoint protein RAD17; FLJ41520; hRad17; R24L; RAD1 homolog; RAD17; RAD17 checkpoint clamp loader component; RAD17 homolog; RAD17 homolog (S. pombe); Rad17-like protein; RAD17SP; RAD24; RF-C activator 1 homolog; RF-C/activator 1 homolog

Synonyms

CCYC; cell cycle checkpoint protein (RAD17); Cell cycle checkpoint protein RAD17; FLJ41520; hRad17; R24L; RAD1 homolog; RAD17; RAD17 checkpoint clamp loader component; RAD17 homolog; RAD17 homolog (S. pombe); Rad17-like protein; RAD17SP; RAD24; RF-C activator 1 homolog; RF-C/activator 1 homolog

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