Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit Monoclonal Antibody (SignalFlexTMPacific BlueTMConjugate)#69600,Cell Signaling Technology (CST),69600

Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (SignalFlexTM Pacific BlueTM Conjugate) detects endogenous levels of ribosomal protein S6 only when phosphorylated at serine 235 and 236.

Host

Rabbit

Reactivity

Human, Mouse, Rat, Monkey

Conjugate

SignalFlexTMPacific BlueTMConjugate

Platform ID

BAB641437693

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NamePhospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit Monoclonal Antibody (SignalFlexTMPacific BlueTMConjugate)#69600
Cat. No.69600
Accession NumberP62753
Gene ID (Entrez)62753, 6194
HostRabbit
SensitivityEndogenous
ReactivityHuman, Mouse, Rat, Monkey
ConjugationSignalFlexTMPacific BlueTMConjugate
ImmunogenIgG
FormulationSignalFlexTM conjugates are produced using highly validated Cell Signaling Technology®primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays.Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.
StorageSupplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C.Do not aliquot the antibody. Protect from light. Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of S6 protein (4,5).Dufner, A. and Thomas, G. (1999)Exp Cell Res253, 100-9.Peterson, R.T. and Schreiber, S.L. (1998)Curr Biol8, R248-50.Jefferies, H.B. et al. (1997)EMBO J16, 3693-704.Ferrari, S. et al. (1991)J Biol Chem266, 22770-5.Flotow, H. and Thomas, G. (1992)J Biol Chem267, 3074-8.Kotecha, N. et al. (2008)Cancer Cell14, 335-43.Alternate Names40S ribosomal protein S6; Phosphoprotein NP33; ribosomal protein S6; RPS6; RS6; S6; S6 ribosomal protein; Small ribosomal subunit protein eS6

Synonyms

40S ribosomal protein S6; Phosphoprotein NP33; ribosomal protein S6; RPS6; RS6; S6; S6 ribosomal protein; Small ribosomal subunit protein eS6

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