Phospho-SMAD2 (Ser465/Ser467) (E8F3R) Rabbit Monoclonal Antibody (BSA and Azide Free)#94610,Cell Signaling Technology (CST),94610

Phospho-SMAD2 (Ser465/Ser467) (E8F3R) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of Smad2 protein when phosphorylated at Ser465 and Ser467.

Host

Rabbit

Reactivity

Human, Mouse, Rat

Platform ID

BAB625434114

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NamePhospho-SMAD2 (Ser465/Ser467) (E8F3R) Rabbit Monoclonal Antibody (BSA and Azide Free)#94610
Cat. No.94610
Accession NumberQ15796
Gene ID (Entrez)15796, 4087
HostRabbit
SensitivityEndogenous
ReactivityHuman, Mouse, Rat
Molecular Weight60
ImmunogenIgG
FormulationThis product is the carrier free version of product #18338. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, pleasecontact us. Optimal dilutions/concentrations should be determined by the end user.BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
StorageStore at -20°C.This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles.A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
Regulatory StatusResearch Use Only

Scientific Background

Members of the SMAD family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of SMADs have been defined: the receptor-regulated SMADs (R-SMADs), which include SMAD1, 2, 3, 5, and 9; the common-mediator SMAD (co-SMAD), SMAD4; and the antagonistic or inhibitory SMADs (I-SMADs), SMAD6 and 7 (1-5). Activated type I receptors associate with specific R-SMADs and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-SMADs dissociate from the receptor and form a heteromeric complex with SMAD4, initiating translocation of the heteromeric SMAD complex to the nucleus. Once in the nucleus, SMADs recruit a variety of DNA binding proteins that function to regulate transcriptional activity (6-8).Heldin, C.H. et al. (1997)Nature390, 465-71.Attisano, L. and Wrana, J.L. (1998)Curr Opin Cell Biol10, 188-94.Derynck, R. et al. (1998)Cell95, 737-40.Massagué, J. (1998)Annu Rev Biochem67, 753-91.Whitman, M. (1998)Genes Dev12, 2445-62.Wrana, J.L. (2000)Sci STKE2000, re1.Attisano, L. and Wrana, J.L. (2002)Science296, 1646-7.Moustakas, A. et al. (2001)J Cell Sci114, 4359-69.Alternate NameshMAD-2; hSMAD2; JV18; JV18-1; MAD homolog 2; Mad protein homolog; Mad-related protein 2; MAD, mothers against decapentaplegic homolog 2; MADH2; MADR2; MGC22139; MGC34440; mother against DPP homolog 2; Mothers against decapentaplegic homolog 2; Mothers against DPP homolog 2; Sma- and Mad-related protein 2; SMAD 2; SMAD family member 2; SMAD, mothers against DPP homolog 2; SMAD2

Synonyms

hMAD-2; hSMAD2; JV18; JV18-1; MAD homolog 2; Mad protein homolog; Mad-related protein 2; MAD, mothers against decapentaplegic homolog 2; MADH2; MADR2; MGC22139; MGC34440; mother against DPP homolog 2; Mothers against decapentaplegic homolog 2; Mothers against DPP homolog 2; Sma- and Mad-related protein 2; SMAD 2; SMAD family member 2; SMAD, mothers against DPP homolog 2; SMAD2

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