Phospho-Src (Tyr530) (F8Z1Z) Rabbit Monoclonal Antibody#42251,Cell Signaling Technology (CST),42251

Phospho-Src (Tyr530) (F8Z1Z) Rabbit Monoclonal Antibody detects endogenous levels of Src only when phosphorylated at Tyr530. This antibody may cross-react with other Src family members such as Yes, Fyn, Fgr, and Yrk when phosphorylated at the equivalent sites.

Host

Rabbit

Reactivity

Human, Mouse, Rat

Application

Western Blotting: 1:1000 Fluorescent Western: 1:1000 Simple WesternTM: 1:50 - 1:250

Platform ID

BAB563052807

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NamePhospho-Src (Tyr530) (F8Z1Z) Rabbit Monoclonal Antibody#42251
Cat. No.42251
Accession NumberP12931
Gene ID (Entrez)12931, 6714
HostRabbit
SensitivityEndogenous
ReactivityHuman, Mouse, Rat
ApplicationWestern Blotting: 1:1000 Fluorescent Western: 1:1000 Simple WesternTM: 1:50 - 1:250
Molecular Weight60
ImmunogenIgG
FormulationSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C.Do not aliquot the antibody.
StorageSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C.Do not aliquot the antibody.
Regulatory StatusResearch Use Only

Scientific Background

The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr530 in the carboxy-terminal tail by Csk renders the enzyme less active (2).Thomas, S.M. and Brugge, J.S. (1997)Annu Rev Cell Dev Biol13, 513-609.Hunter, T. (1987)Cell49, 1-4.Alternate NamesASV; c-SRC; p60-Src; pp60c-src; Proto-oncogene c-Src; Proto-oncogene tyrosine-protein kinase Src; protooncogene SRC, Rous sarcoma; SRC; SRC proto-oncogene, non-receptor tyrosine kinase; SRC1; THC6; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)

Synonyms

ASV; c-SRC; p60-Src; pp60c-src; Proto-oncogene c-Src; Proto-oncogene tyrosine-protein kinase Src; protooncogene SRC, Rous sarcoma; SRC; SRC proto-oncogene, non-receptor tyrosine kinase; SRC1; THC6; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)

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