Phospho-p53 (Ser15) (E9Y4U) Rabbit Monoclonal Antibody#82530,Cell Signaling Technology (CST),82530

The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). p53 is phosphorylated at multiple sitesin vivoand by several different protein kinasesin vitro(2,3). DNA damage induces phosphorylation of p53 at Ser15 and Ser20 and leads to a reduced interaction between p53 and its negative regulator, the oncoprotein MDM2 (4). MDM2 inhibits p53 accumulation by targeting it for ubiquitination and proteasomal degradation (5,6). p53 can be phosphorylated by ATM, ATR, and DNA-PK at Ser15 and Ser37. Phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and activation of p53 in response to DNA damage (4,7). Chk2 and Chk1 can phosphorylate p53 at Ser20, enhancing its tetramerization, stability, and activity (8,9). p53 is phosphorylated at Ser392in vivo(10,11) and by CAKin vitro(11). Phosphorylation of p53 at Ser392 is increased in human tumors (12) and has been reported to influence the growth suppressor function, DNA binding, and transcriptional activation of p53 (10,13,14). p53 is phosphorylated at Ser6 and Ser9 by CK1δ and CK1ε bothin vitroandin vivo(13,15). Phosphorylation of p53 at Ser46 regulates the ability of p53 to induce apoptosis (16). Acetylation of p53 is mediated by p300 and CBP acetyltransferases. Inhibition of deacetylation suppressing MDM2 from recruiting HDAC1 complex by p19 (ARF) stabilizes p53. Acetylation appears to play a positive role in the accumulation of p53 protein in stress response (17). Following DNA damage, human p53 becomes acetylated at Lys382 (Lys379 in mouse)in vivoto enhance p53-DNA binding (18). Deacetylation of p53 occurs through interaction with the SIRT1 protein, a deacetylase that may be involved in cellular aging and the DNA damage response (19).Levine, A.J. (1997)Cell88, 323-31.Meek, D.W. (1994)Semin Cancer Biol5, 203-10.Milczarek, G.J. et al. (1997)Life Sci60, 1-11.Shieh, S.Y. et al. (1997)Cell91, 325-34.Chehab, N.H. et al. (1999)Proc Natl Acad Sci U S A96, 13777-82.Honda, R. et al. (1997)FEBS Lett420, 25-7.Tibbetts, R.S. et al. (1999)Genes Dev13, 152-7.Shieh, S.Y. et al. (1999)EMBO J18, 1815-23.Hirao, A. et al. (2000)Science287, 1824-7.Hao, M. et al. (1996)J Biol Chem271, 29380-5.Lu, H. et al. (1997)Mol Cell Biol17, 5923-34.Ullrich, S.J. et al. (1993)Proc Natl Acad Sci U S A90, 5954-8.Kohn, K.W. (1999)Mol Biol Cell10, 2703-34.Lohrum, M. and Scheidtmann, K.H. (1996)Oncogene13, 2527-39.Knippschild, U. et al. (1997)Oncogene15, 1727-36.Oda, K. et al. (2000)Cell102, 849-62.Ito, A. et al. (2001)EMBO J20, 1331-40.Sakaguchi, K. et al. (1998)Genes Dev12, 2831-41.Solomon, J.M. et al. (2006)Mol Cell Biol26, 28-38.Alternate NamesAntigen NY-CO-13; BCC7; BMFS5; Cellular tumor antigen p53; FLJ92943; LFS1; mutant tumor protein 53; P53; p53 antigen; p53 transformation suppressor; p53 tumor suppressor; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein 53; tumor protein p53; Tumor suppressor p53; tumor supressor p53

Antigen NY-CO-13; BCC7; BMFS5; Cellular tumor antigen p53; FLJ92943; LFS1; mutant tumor protein 53; P53; p53 antigen; p53 transformation suppressor; p53 tumor suppressor; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein 53; tumor protein p53; Tumor suppressor p53; tumor supressor p53