PhosphoPlus^®beta-Catenin (Ser675) Antibody Duet#11887,Cell Signaling Technology (CST),11887

β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).Cadigan, K.M. and Nusse, R. (1997)Genes Dev11, 3286-3305.Wodarz, A. and Nusse, R. (1998)Annu Rev Cell Dev Biol14, 59-88.Polakis, P. (1999)Curr Opin Genet Dev9, 15-21.Amit, S. et al. (2002)Genes Dev16, 1066-76.Liu, C. et al. (2002)Cell108, 837-47.Yanagawa, S. et al. (2002)EMBO J21, 1733-42.Yost, C. et al. (1996)Genes Dev10, 1443-54.Morin, P.J. et al. (1997)Science275, 1787-90.