Purified anti-53BP1 Antibody, 53BP1, W17184B,BioLegend,933001

This clone recognizes both human and mouse 53BP1.This clone was tested for ICC using both Triton X-100 and methanol permeabilization with both methods showing correct staining.This clone failed IP testing.Predicted MW of 53BP1 is 214 kD but the observed MW is ~350kD. An identical observed MW was noted when comparing to other control antibodies on the market. Similar differences in predicted and observed molecular weights are reported with other antibodies on the market.

Host

Rat

Reactivity

Human, Mouse

Application

WB -Quality testedICC -Verified

Platform ID

BAB955365786

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NamePurified anti-53BP1 Antibody, 53BP1, W17184B
Cat. No.933001
HostRat
RRIDAB_2820201 (BioLegend Cat. No. 933001)AB_2820202 (BioLegend Cat. No. 933002)
IsotypeRat IgG2a, κ
ReactivityHuman, Mouse
ApplicationWB -Quality testedICC -Verified
ClonalityMonoclonal
Clone NumberW17184B
Concentration0.5 mg/mL
Target53BP1
ImmunogenPartial recombinant human 53BP1 protein.
PurityThe antibody was purified by affinity chromatography.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C.
Regulatory StatusResearch Use Only

Scientific Background

p53-binding protein 1 (53BP1) was first discovered as an interacting partner of p53 tumor suppressor protein in a yeast two-hybrid screening. 53BP1 binds to p53 through a BRCA1 C-terminal (BRCT) domain and enhances p53-dependent transcriptional activation. 53BP1 has been studied extensively for its function in the regulation of DNA double-strand break (DSB) repair pathway. Additionally, it promotes non-homologous end joining (NHEJ) while inhibiting homologous recombination (HR) by preventing DNA end resection. Upon DSB, the ubiquitin ligase RNF168 ubiquitylates lysine 15 on histone H2A/H2AX, which serves as a binding site for 53BP1. 53BP1 is then phosphorylated by ATM, which facilitates the recruitment of downstream effector proteins, including PTIP and RIF1. 53BP1-mediated NHEJ is essential for V(D)J recombination and class switch recombination.

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