Purified anti-APEX1 Antibody, APEX1, 1518CT337.123.86.269.232,BioLegend,605051
When using this clone for ICC, we recommend using PFA + methanol or methanol only fix-perm conditions. We do not recommend using PFA + Triton X-100 due to non-specific staining.When testing this clone for ICC, this antibody produced filamentous, extranuclear staining in some cells. This pattern was also observed with a control antibody generated using a different APEX1 immunogen, consistent with this staining being APEX1 dependent.
Host
Mouse
Reactivity
Human, Mouse
Application
WB - Quality testedIHC-P, ICC - Verified
Platform ID
BAB853825813
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
Email:
Specifications
Scientific Background
Apurinic-apyridimic endonuclease-1, or APEX1, is a multifunctional enzyme required for the repair of DNA damaged by both endogenous and exogenous agents. It also exerts oncogenic function as a redox signaling protein by reductively stimulating the DNA-binding activity of transcription factors that drive cancer progression, including c-Jun, AP-1, NF-κB, p53, and HIF1α. APEX1 expression level and subcellular localization are positively correlated with cancer progression, and elevated APEX1 expression may also confer resistance to DNA-damaging chemotherapeutic agents.
Category Paths
- Products>Primary Antibodies>IHC Antibodies
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