Purified anti-BCAT1 Antibody, BCAT1, W18024A,BioLegend,938002
This clone failed to immunoprecipitate BCAT1 from NTERA-2 lysates.This clone was tested for immunocytochemistry on NTERA-2 cells fixed with 4% PFA and permeabilized with either methanol or Triton X-100. Both methods resulted in dim staining of BCAT1. We therefore do not recommend the clone for this application.
Host
Rat
Reactivity
Human
Application
WB - Quality tested
Platform ID
BAB143509227
BioLegend
Contact
Tel: 1-858-455-9588
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Product Specifications
Scientific Background
Specifications
NamePurified anti-BCAT1 Antibody, BCAT1, W18024A
Cat. No.938002
HostRat
RRIDAB_2876757 (BioLegend Cat. No. 938001)AB_2876757 (BioLegend Cat. No. 938002)
IsotypeRat IgG2a, κ
ReactivityHuman
ApplicationWB - Quality tested
ClonalityMonoclonal
Clone NumberW18024A
Concentration0.5 mg/mL
TargetBCAT1
ImmunogenFull-length recombinant human BCAT1 protein
PurityThe antibody was purified by affinity chromatography.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
StorageThe antibody solution should be stored undiluted between 2°C and 8°C.
Regulatory StatusResearch Use Only
Scientific Background
BCAT1 is an aminotransferase that catalyzes the catabolism of the branched-chain amino acids leucine, isoleucine, and valine, making it an important regulator of the TCA cycle. BCAT1 also plays a role in cell cycle progression, differentiation, and apoptosis. Clinical disorders involved with mutations in theBCAT1gene include hypervalinemia and hyperleucine-isoleucinemia.
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