Purified anti-E coli RNA Polymerase beta Antibody anti-RNA Polymerase beta - 8RB13,BioLegend,663905

Clone 8RB13 is extremely useful for detecting or purifying core RNAP from a broad range of bacteria. The purified polymerase is highly active forin vitrotranscription.Additional reported applications (for the relevant formats) include: immunoprecipitation3. The epitope of clone 8RB13 has been mapped to the sequence PEEKLLRAIFGEKAS3.

Host

Mouse

Reactivity

E. coli

Application

WB - Quality testedELISA, IP - Reported in the literature, not verified in house

Platform ID

BAB260997863

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Product Specifications
Scientific Background

Specifications

NamePurified anti-E coli RNA Polymerase beta Antibody anti-RNA Polymerase beta - 8RB13
Cat. No.663905
HostMouse
RRIDAB_2566583 (BioLegend Cat. No. 663905)
IsotypeMouse IgG2b, κ
ReactivityE. coli
ApplicationWB - Quality testedELISA, IP - Reported in the literature, not verified in house
ClonalityMonoclonal
Clone Number8RB13
Concentration1.0 mg/ml
TargetRNA Polymerase beta
ImmunogenPurified recombinantE. coliRNA polymerase β subunit.
PurityThe antibody was purified by affinity chromatography.
Formulation0.22 µm filtered protein solution is in phosphate-buffered solution, pH 7.2.
StorageThis antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label orCoAto find the proper storage conditions.
Regulatory StatusResearch Use Only

Scientific Background

RNA polymerase (RNAP or RNApol) is an enzyme that produces primary transcript RNA. The β subunit is the second-largest subunit, and is encoded by the roB gene. RNA polymerase β is a subunit which, along wtih α (rpoC), limits core polymerase expression.

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