Purified anti-GD3 (MEL-1) Recombinant Antibody,BioLegend,638851
For use of this antibody (clone R24.Rec) in immunocytochemistry (ICC), we recommend fixation with Fixation Buffer (Cat. No. 420801), followed by permeabilization with 0.5% Triton-X. We do not recommend fixation with Fixation Buffer (Cat. No. 420801) followed by permeabilization with ice-cold methanol, nor fixation/permeabilization with ice-cold methanol alone.For use of this antibody (clone R24.Rec) in intracellular flow cytometry (ICFC), we recommend fixation/permeabilization with Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803).This antibody (clone R24.Rec) is not suitable for use in immunohistochemistry with formalin-fixed, paraffin-embedded tissues (IHC-P).
Host
Mouse
Reactivity
Human
Application
IHC-F - Quality testedICC, ICFC - Verified
Platform ID
BAB208400593
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
Email:
Product Specifications
Scientific Background
Specifications
NamePurified anti-GD3 (MEL-1) Recombinant Antibody
Cat. No.638851
HostMouse
RRIDAB_3662322 (BioLegend Cat. No. 638851)AB_3662322 (BioLegend Cat. No. 638852)
IsotypeMouse IgG1, κ
ReactivityHuman
ApplicationIHC-F - Quality testedICC, ICFC - Verified
ClonalityRecombinant
Clone NumberR24.Rec
Concentration0.5 mg/mL
TargetGD3
ImmunogenThis antibody was raised against SK-MEL-28 melanoma cell line.
PurityThe antibody was purified by affinity chromatography.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
StorageThe antibody solution should be stored undiluted between 2°C and 8°C.
Regulatory StatusResearch Use Only
Scientific Background
GD3, also known as MEL-1, is a ganglioside found in almost all melanomas and is an important biomarker for malignant melanoma. GD3 is found in melanocytes and other cells that originate in the neuroectoderm. Anti-GD3 antibodies have been shown to induce partial remission of tumor growth due to the proliferation of cytotoxic lymphocytes. GD3 is synthesized by the enzyme ST8SIA1.
Category Paths
- Products>Primary Antibodies>IHC Antibodies
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