Purified anti-HAGH Antibody, HAGH, W22099D,BioLegend,682951

This antibody (clone W22099D) is not suitable for immunocytochemistry (ICC).For immunohistochemistry on formalin-fixed parafilm-embedded tissue (IHC-P), the recommended antigen retrieval is either Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704) or Citrate Buffer (Cat. No.420902).

Host

Rat

Reactivity

Human

Application

WB - Quality testedIHC-P - Verified

Platform ID

BAB649356419

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NamePurified anti-HAGH Antibody, HAGH, W22099D
Cat. No.682951
HostRat
RRIDAB_3662337 (BioLegend Cat. No. 682951)AB_3662337 (BioLegend Cat. No. 682952)
IsotypeRat IgG2a, κ
ReactivityHuman
ApplicationWB - Quality testedIHC-P - Verified
ClonalityMonoclonal
Clone NumberW22099D
Concentration0.5 mg/mL
TargetHAGH
ImmunogenRecombinant fragment of human HAGH (GLO2)
PurityThe antibody was purified by affinity chromatography.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
StorageThe antibody solution should be stored undiluted between 2°C and 8°C.
Regulatory StatusResearch Use Only

Scientific Background

Hydroxyacylglutathione hydrolase, mitochondrial or GLO2, is integral to cellular detoxification and metabolic regulation. It operates within the glyoxalase system, a critical pathway for neutralizing methylglyoxal, a highly reactive and cytotoxic byproduct of glycolysis. The glyoxalase system includes two primary enzymes: Lactoylglutathione lyase (GLO1) and HAGH/GLO2. GLO1 catalyzes the reaction between methylglyoxal and glutathione (GSH) to form S-D-lactoylglutathione. Subsequently, HAGH/GLO2 hydrolyzes S-D-lactoylglutathione to form D-lactate and regenerates GSH. This detoxification is crucial as methylglyoxal can cause significant cellular damage by glycation, which impairs the function of proteins, nucleic acids, and lipids. By mitigating this damage, HAGH helps protect cellular components and maintain cellular integrity.

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