Purified anti-IDH2 Antibody, IDH2, KrMab-3,BioLegend,946002
This clone was tested for ICC using 4% PFA-fixed HepG2 cells permeabilized with either methanol or Triton X-100. While staining was observed with both methods, Triton X-100 permeabilization resulted in stronger staining.
Host
Mouse
Reactivity
Human, Mouse
Application
WB - Quality testedICC, IP - Verified
Platform ID
BAB055251611
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
Email:
Specifications
Scientific Background
Isocitrate dehydrogenase 2 (IDH2) is a mitochondrial enzyme that is directly involved in the TCA cycle. The primary role of IDH2 is to convert isocitrate to 2-ketoglutarate (α-KG). The mutated isoform of IDH2 has been reported in glioblastomas, AML, and other cancers. The gain of function mutation in IDH2 leads to the accumulation of oncometabolite 2-hydroxyglutarate (2HG), which competitively inhibits HIF Prolyl Hydroxylase 2 (PHD2). This results in the stabilization and accumulation of hypoxia-inducible factors (HIF). The downstream effects of this mutation promote epigenetic changes and impaired cellular differentiation.
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