Purified anti-LAT1/SLC7A5 Antibody, LAT1/SLC7A5, W22208B,BioLegend,688751

For use of this antibody in Immunocytochemistry (ICC), we recommend fixation/permeabilization using either PFA-Methanol or Methanol only. We do not recommend PFA-Triton X-100 for fixation/permeabilization.For use of this antibody in immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902), or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).For use of this antibody in intracellular Flow Cytometry (ICFC), we recommend fixation/permeabilization using True-Phos™ Perm Buffer (Cat. No. 425401).

Host

Rat

Reactivity

Human

Application

WB - Quality testedICC, ICFC, IHC-P - Verified

Platform ID

BAB149606299

BioLegend

Headquarters

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Contact

Tel: 1-858-455-9588
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Product Specifications
Scientific Background

Specifications

NamePurified anti-LAT1/SLC7A5 Antibody, LAT1/SLC7A5, W22208B
Cat. No.688751
HostRat
RRIDAB_3683498 (BioLegend Cat. No. 688751)AB_3683498 (BioLegend Cat. No. 688752)
IsotypeRat IgG2b, κ
ReactivityHuman
ApplicationWB - Quality testedICC, ICFC, IHC-P - Verified
ClonalityMonoclonal
Clone NumberW22208B
Concentration0.5 mg/mL
TargetLAT1/SLC7A5
ImmunogenFragment of recombinant Human LAT1/SLC7A5
PurityThe antibody was purified by affinity chromatography.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
StorageThe antibody solution should be stored undiluted between 2°C and 8°C.
Regulatory StatusResearch Use Only

Scientific Background

L-Type Amino Acid Transporter 1 (LAT1) is crucial for transporting large neutral amino acids like leucine, isoleucine, and phenylalanine across cell membranes, playing a significant role in cellular metabolism and nutrient supply. LAT1 is highly expressed in the blood-brain barrier, where it facilitates the delivery of essential amino acids to the brain. Its regulation involves various mechanisms, including transcriptional and post-transcriptional controls, which ensure its activity aligns with cellular needs. Dysregulation of LAT1 is associated with several diseases, including cancer, where it supports tumor growth by supplying amino acids necessary for rapid cell proliferation. Additionally, LAT1 is implicated in neurological disorders like Alzheimer's and Parkinson's diseases, where it affects the transport of therapeutic agents across the blood-brain barrier.

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