Purified anti-LEF1 Antibody, LEF1, W17021C,BioLegend,621052

LEF-1 is known to have 7 isoforms.WB (Western blotting) or IHC-P (Immunohistochemistry FFPE) is not suggested for this product.For WB, IP (immunoprecipitation) or ICC (immunocytochemistry) applications, please refer to clones 15H5A18 (Cat. No. 653101/653102) or W17155A (Cat. No. 616001/616002).For ICFC (intracellular flowcytometry), True-Phos™ Perm Buffer (Cat. No. 425401) is suggested for permeabilization.

Host

Rat

Reactivity

Human

Application

ICFC - Quality tested

Platform ID

BAB704641134

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NamePurified anti-LEF1 Antibody, LEF1, W17021C
Cat. No.621052
HostRat
RRIDAB_3083406 (BioLegend Cat. No. 621051)AB_3083406 (BioLegend Cat. No. 621052)
IsotypeRat IgG2a, λ
ReactivityHuman
ApplicationICFC - Quality tested
ClonalityMonoclonal
Clone NumberW17021C
Concentration0.5 mg/mL
TargetLEF1
ImmunogenPartial recombinant human LEF1 protein corresponding to amino acid residues 116-283
PurityThe antibody was purified by affinity chromatography.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
StorageThe antibody solution should be stored undiluted between 2°C and 8°C.
Regulatory StatusResearch Use Only

Scientific Background

Lymphoid Enhancing Factor 1 (LEF1) is a transcription factor belonging to the TCF/LEF family. LEF1 was first identified as a protein binding to TCR-α enhancer and upregulates expression of T-cell antigen receptor α chain. LEF1 is involved in Wnt signaling pathways as a ubiquitous regulator. During Wnt signaling, translocation of β-catenin from the cytosol to the nucleus results in elevation of transcriptional activity of LEF1, which subsequently regulates multiple downstream gene expression. LEF1 is implicated as a transcriptional regulator in the transformation of cancer cells in various diseases such as colonic adenocarcinoma. Due to its role in Wnt/β-catenin signaling pathway, LEF1 has been indicated as a facilitator of EMT and potential biomarker for various cancers.

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