Purified anti-LEF1 Antibody, LEF1, W17155A,BioLegend,616001
W17155A recognizes multiple isoforms of LEF1.When tested for ICC, W17155A failed to stain PFA-fixed Jurkat cells permeabilized with methanol. We recommend Triton X-100 permeabilization.
Host
Rat
Reactivity
Human
Application
WB -Quality testedICC, IP -Verified
Platform ID
BAB892784575
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
Email:
Product Specifications
Scientific Background
Specifications
NamePurified anti-LEF1 Antibody, LEF1, W17155A
Cat. No.616001
HostRat
RRIDAB_2814460 (BioLegend Cat. No. 616001)AB_2814461 (BioLegend Cat. No. 616002)
IsotypeRat IgG2b, κ
ReactivityHuman
ApplicationWB -Quality testedICC, IP -Verified
ClonalityMonoclonal
Clone NumberW17155A
Concentration0.5 mg/ml
TargetLEF1
ImmunogenPartial recombinant human LEF1 protein corresponding to amino acid residues 116-283
PurityThe antibody was purified by affinity chromatography.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C.
Regulatory StatusResearch Use Only
Scientific Background
Lymphoid Enhancing Factor 1 (LEF1) is a transcription factor belonging to the TCF/LEF family. LEF1 was first identified as a protein binding to TCR-α enhancer and upregulates expression of T-cell antigen receptor α chain. LEF1 is involved in Wnt signaling pathways as a ubiquitous regulator. During Wnt signaling, translocation of β-catenin from the cytosol to the nucleus results in elevation of transcriptional activity of LEF1, which subsequently regulates multiple downstream genes.
Category Paths
Request a product
Please provide the required information below so that we can quickly source your products.