Purified anti-PIEZO1 Antibody, PIEZO1, W17241A,BioLegend,602752
When testing this clone for western blot, we observed a considerable loss of signal with lysates that were boiled in sample buffer compared to samples that were heated to 37°C. This is likely due to high temperatures causing aggregation of PIEZO1, which is a multi-pass transmembrane domain protein.This clone was tested for immunocytochemistry on HeLa cells fixed with 4% PFA and permeabilized with either methanol or Triton X-100. Both methods resulted in high non-specific staining.
Host
Rat
Reactivity
Human, Mouse
Application
IHC-P - Quality testedWB - Verified
Platform ID
BAB830703124
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
Email:
Specifications
Scientific Background
PIEZO1 and its paralog PIEZO2 are members of a non-selective cationic mechanosensitive channel family first discovered in a neuronal cell line through mechanical stimulation. It responds to mechanical forces such as static pressure, shear stress, and membrane stretch events by allowing the efflux of calcium ions from the ER lumen into the cytosol. The increase in cytosolic calcium results in phosphorylation of ERK1/2 and a commensurate induction of downstream mitotic genes, and can also promote prostate cancer development via activation of the Akt/mTOR signaling axis. PIEZO1 is overexpressed in many tumors and is a key regulator of tumor stiffness.
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