Purified anti-SMAD2 Antibody, SMAD2, W21150C,BioLegend,624851

This clone cross-reacts with mouse.For intracellular flow cytometric staining with this clone, we recommended using Fixation Buffer (Cat. No. 420801), True-Phos™ Perm Buffer (Cat. No. 425401), Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002) or Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803). True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) is not applicable.We do not recommend this product for use in immunocytochemistry (ICC).

Host

Rat

Reactivity

Human, Mouse

Application

WB - Quality testedICFC - Verified

Platform ID

BAB336450364

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

Email:

Product Specifications
Scientific Background

Specifications

NamePurified anti-SMAD2 Antibody, SMAD2, W21150C
Cat. No.624851
HostRat
RRIDAB_3097247 (BioLegend Cat. No. 624851)AB_3097247 (BioLegend Cat. No. 624852)
IsotypeRat IgG2b, κ
ReactivityHuman, Mouse
ApplicationWB - Quality testedICFC - Verified
ClonalityMonoclonal
Clone NumberW21150C
Concentration0.5 mg/mL
TargetSMAD2
ImmunogenRecombinant human SMAD2 protein
PurityThe antibody was purified by affinity chromatography.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
StorageThe antibody solution should be stored undiluted between 2°C and 8°C.
Regulatory StatusResearch Use Only

Scientific Background

SMAD2 is a potent regulator of different cellular processes such as apoptosis, differentiation, and cell proliferation. SMAD2 is associated with SARA protein and the TGF-β receptor. SMAD2 gets phosphorylated by the TGF-β receptor in response to TGF-β signaling, which induces its dissociation from SARA and association with SMAD4 followed by translocation into the nucleus. In the nucleus, the SMAD2/SMAD4 protein complex participates in gene regulation by forming a transcription repressor complex in association with other cofactors.

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