Purified anti-STIM1 Antibody, STIM1, W18094A,BioLegend,621702
When testing W18094A testing for ICC, we observed poor staining of PFA-fixed cells that were permeabilized with methanol.When testing W18094A for WB, a minor non-specific band of ~50 kD was observed by WB at higher antibody concentrations.
Host
Rat
Reactivity
Human
Application
WB -Quality testedICC, IP -Verified
Platform ID
BAB206290342
BioLegend
Contact
Tel: 1-858-455-9588
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Product Specifications
Scientific Background
Specifications
NamePurified anti-STIM1 Antibody, STIM1, W18094A
Cat. No.621702
HostRat
RRIDAB_2814470 (BioLegend Cat. No. 621701)AB_2814471 (BioLegend Cat. No. 621702)
IsotypeRat IgG2b, λ
ReactivityHuman
ApplicationWB -Quality testedICC, IP -Verified
ClonalityMonoclonal
Clone NumberW18094A
Concentration0.5 mg/ml
TargetSTIM1
ImmunogenPartial recombinant human STIM1 protein corresponding to amino acid residues 436-685
PurityThe antibody was purified by affinity chromatography.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C.
Regulatory StatusResearch Use Only
Scientific Background
Stromal Interaction Molecule 1, or STIM1 is a single pass transmembrane, resident ER protein that facilitates extracellular calcium uptake following the depletion of intracellular stores. STIM1 directly senses loss of ER-lumenal calcium via an EF hand domain; elevated ER calcium levels stimulate redistribution of STIM1 to close proximity to the plasma mebrane, where it activates ORAI1, a pore component of the store-operated calcium entry system.
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