Purified anti-Spt16 FACT140 complex Antibody anti-Spt16 (FACT140 complex) - 8D2,BioLegend,607001
25 µg, 100 µg of Go-ChIP-Grade™ Purified Antibody can be used for 2-4, 11-16 immunoprecipitations, respectively, at the recommended dilution. ICC positive in HeLa Cells.
Host
Mouse
Reactivity
Human, Mouse
Application
WB -Quality testedICC, ChIP -VerifiedIP -Reported in the literature, not verified in house
Platform ID
BAB510523091
BioLegend
Contact
Tel: 1-858-455-9588
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Product Specifications
Scientific Background
Specifications
NamePurified anti-Spt16 FACT140 complex Antibody anti-Spt16 (FACT140 complex) - 8D2
Cat. No.607001
HostMouse
RRIDAB_315688 (BioLegend Cat. No. 607001)AB_315689 (BioLegend Cat. No. 607002)
IsotypeMouse IgG2a, κ
ReactivityHuman, Mouse
ApplicationWB -Quality testedICC, ChIP -VerifiedIP -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone Number8D2
Concentration0.5 mg/ml
TargetSpt16 FACT140 complex
ImmunogenRecombinant (full length)
PurityThe antibody was purified by affinity chromatography.
FormulationThis antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageUpon receipt, store between 2°C and 8°C.
Regulatory StatusResearch Use Only
Scientific Background
Spt16 is a 120-140 kD nuclear protein and a component of the FACT complex (facilitates chromatin transcription). The FACT140 complex is composed of the Spt16 and the SSRP1 proteins. This complex interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation. The 8D2 monoclonal antibody recognizes human Spt16 and has been shown to be useful for Western blotting.
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