Purified anti-Wnt7a Antibody, Wnt7a, W19053A,BioLegend,949502

The WNT protein family has a highly conserved cysteine-rich structure that associates with the extracellular matrix (ECM) with high affinity through binding to heparin sulfate glycoproteins. They are carried intercellularly by lipoproteins and mediate signaling activity by binding to transmembrane Frizzled receptors and low-density-related proteins (LRP). They elicit a variety of responses through canonical or non-canonical (PCP or Ca2+) pathways. There are two subtypes of WNT7 that have been identified, WNT7a and WNT7b.WNT7a is a secreted cell-surface signaling protein that controls a set of pathways that act on cell fate determination and tissue patterning during embryogenesis. It is known that WNT7a can modulate the development of the uterus, cerebellum and limbs, while WNT7b is important in lung and placental development. The human amino acid sequence of WNT7a shares high similarity (99.4%) with mouse WNT7a. Signals transduced through WNT7a and its receptor are critical for female organ development, maintaining levels of female sex hormones and the development of oviduct and uterus. Receptors for WNT7a include frizzled-5, frizzled-7 and frizzled-10. WNT7a binds to frizzled-7 to induce signaling through the SKT/mTOR pathway in order to further modulate myofibers in a G-protein dependent PI3K pathway.During muscle cell regeneration, WNT7a in the ECM along with transiently raised fibronectin levels in the stem cell niche can bind the frizzled-7/Sdc4 receptor complex and synergistically induce symmetric divisions of satellite stem cells. Also, in neurogenesis and chondrogenesis, WNT7a contributes to regulating and maintaining the level of lineage differentiation factors required to promote apoptosis/cell death through the binding of different receptors and interactions with ECM proteins.