Purified anti-human CD3 Antibody anti-CD3 - HIT3a,BioLegend,300301
Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and activation of T lymphocytes4-7. The HIT3a antibody is able to stimulate T cell activation. The LEAF™purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300314). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300332) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Host
Mouse
Reactivity
Human
Application
FC -Quality testedIP, IHC-F, Activ -Reported in the literature, not verified in house
Platform ID
BAB296680727
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
Email:
Product Specifications
Scientific Background
Specifications
NamePurified anti-human CD3 Antibody anti-CD3 - HIT3a
Cat. No.300301
HostMouse
RRIDAB_314037 (BioLegend Cat. No. 300301)AB_314038 (BioLegend Cat. No. 300302)
IsotypeMouse IgG2a, κ
ReactivityHuman
ApplicationFC -Quality testedIP, IHC-F, Activ -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberHIT3a
Concentration0.5 mg/ml
TargetCD3
PurityThe antibody was purified by affinity chromatography.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C.
Regulatory StatusResearch Use Only
Scientific Background
CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.
Category Paths
- Products>Primary Antibodies>IHC Antibodies
Request a product
Please provide the required information below so that we can quickly source your products.