Purified anti-human CD3 Antibody anti-CD3 - UCHT1,BioLegend,300402
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
Host
Mouse
Reactivity
Human
Application
FC -Quality testedCyTOF®,IHC-F- VerifiedIP, Activ, WB -Reported in the literature, not verified in house
Platform ID
BAB224292961
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
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Product Specifications
Scientific Background
Specifications
NamePurified anti-human CD3 Antibody anti-CD3 - UCHT1
Cat. No.300402
HostMouse
RRIDAB_314055 (BioLegend Cat. No. 300401)AB_314056 (BioLegend Cat. No. 300402)
IsotypeMouse IgG1, κ
ReactivityHuman
ApplicationFC -Quality testedCyTOF®,IHC-F- VerifiedIP, Activ, WB -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberUCHT1
Concentration0.5 mg/ml
TargetCD3
PurityThe antibody was purified by affinity chromatography.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C.
Regulatory StatusResearch Use Only
Scientific Background
CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.
Category Paths
- Products>Primary Antibodies>IHC Antibodies
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