Purified anti-human CD4 Antibody anti-CD4 - RPA-T4,BioLegend,300501

The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections3,4,5, blocking of T cell activation1,2, and spatial biology (IBEX)10,11. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300569 - 300574).

Host

Mouse

Reactivity

Human

Application

FC -Quality testedCyTOF®- VerifiedActiv, Block, IHC-Reported in the literature, not verified in house

Platform ID

BAB899992378

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NamePurified anti-human CD4 Antibody anti-CD4 - RPA-T4
Cat. No.300501
HostMouse
RRIDAB_314069 (BioLegend Cat. No. 300501)AB_314070 (BioLegend Cat. No. 300502)
IsotypeMouse IgG1, κ
ReactivityHuman
ApplicationFC -Quality testedCyTOF®- VerifiedActiv, Block, IHC-Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberRPA-T4
Concentration0.5 mg/ml
TargetCD4
PurityThe antibody was purified by affinity chromatography.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C.
Regulatory StatusResearch Use Only

Scientific Background

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules, and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.

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