Purified anti-human CD45 Antibody anti-CD45 - HI30,BioLegend,304001

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections9, inhibition of CD45 functions4, immunofluorescence11, Western blotting3, and spatial biology (IBEX)16,17.It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.

Host

Mouse

Reactivity

Human

Application

FC -Quality testedCyTOF®, IHC-P -VerifiedWB, IHC-F, ICC -Reported in the literature, not verified in house

Platform ID

BAB419044493

BioLegend

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Contact

Tel: 1-858-455-9588
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Product Specifications
Scientific Background

Specifications

NamePurified anti-human CD45 Antibody anti-CD45 - HI30
Cat. No.304001
HostMouse
RRIDAB_314389 (BioLegend Cat. No. 304001)AB_314390 (BioLegend Cat. No. 304002)
IsotypeMouse IgG1, κ
ReactivityHuman
ApplicationFC -Quality testedCyTOF®, IHC-P -VerifiedWB, IHC-F, ICC -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberHI30
Concentration0.5 mg/ml
TargetCD45
PurityThe antibody was purified by affinity chromatography.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C.
Regulatory StatusResearch Use Only

Scientific Background

CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

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