Purified anti-human HLA-A,B,C Antibody anti-HLA-A,B,C - W6/32,BioLegend,311402

Clone W6/32 recognizes residues in the N terminus of the human ß2-microglobulin molecule21.Additional reported applications (for the relevant formats) include: immunoprecipitaton2, Western blotting (non-reducing)3, immunohistochemical staining of acetone-fixed frozen tissue sections4,5, blocking6,7, inhibition of NK cell-mediated lysis10, and activation8,9. Clone W6/32 has been reported not to be suitable for immunohistochemistry on paraffin sections17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 311428) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Host

Mouse

Reactivity

Human, Cynomolgus, Rhesus

Application

FC -Quality testedActiv, Block, IHC-F, IP, WB -Reported in the literature, not verified in house

Platform ID

BAB400530855

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NamePurified anti-human HLA-A,B,C Antibody anti-HLA-A,B,C - W6/32
Cat. No.311402
HostMouse
RRIDAB_314871 (BioLegend Cat. No. 311402)
IsotypeMouse IgG2a, κ
ReactivityHuman, Cynomolgus, Rhesus
ApplicationFC -Quality testedActiv, Block, IHC-F, IP, WB -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberW6/32
Concentration0.5 mg/ml
TargetHLA-A
PurityThe antibody was purified by affinity chromatography.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C.
Regulatory StatusResearch Use Only

Scientific Background

MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in presentation of antigens to CD8+T cells. They play an important role in cell-mediated immune responses and tumor surveillance.

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