Purified anti-human IL-3 Antibody anti-IL-3 - BVD8-3G11,BioLegend,500502
ELISA1-4or ELISPOT Capture:The purified BVD8-3G11 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated BVD3-1F9 antibody (Cat. No. 500604) as the detecting antibody.Additional reported applications include:Western blotting4and immunoprecipitation4.
Host
Rat
Reactivity
Human
Application
ELISA Capture - Quality testedWB, IP - Reported in the literature, not verified in house
Platform ID
BAB087547366
BioLegend
Contact
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Product Specifications
Scientific Background
Specifications
NamePurified anti-human IL-3 Antibody anti-IL-3 - BVD8-3G11
Cat. No.500502
HostRat
RRIDAB_315103 (BioLegend Cat. No. 500502)
IsotypeRat IgG1, κ
ReactivityHuman
ApplicationELISA Capture - Quality testedWB, IP - Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberBVD8-3G11
Concentration0.5 mg/ml
TargetIL-3
ImmunogenYeast-expressed, recombinant human IL-3
PurityThe antibody was purified by affinity chromatography.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C.
Regulatory StatusResearch Use Only
Scientific Background
IL-3 is a highly species-specific pleiotropic factor produced primarily by activated T cells though also by mast cells, keratinocytes, and astrocytes, which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. The BVD8-3G11 antibody can neutralize the bioactivity of natural or recombinant IL-3.
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