Purified anti-mouse CD45.2 Maxpar Ready Antibody anti-CD45.2 - 104,BioLegend,109843

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4,in vivoandin vitroblocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3.

Host

Mouse

Reactivity

Mouse

Application

FC - Quality testedCyTOF® - Verified

Platform ID

BAB536721774

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Product Specifications
Scientific Background

Specifications

NamePurified anti-mouse CD45.2 Maxpar Ready Antibody anti-CD45.2 - 104
Cat. No.109843
HostMouse
RRIDAB_2563751 (BioLegend Cat. No. 109843)
IsotypeMouse (SJL) IgG2a, κ
ReactivityMouse
ApplicationFC - Quality testedCyTOF® - Verified
ClonalityMonoclonal
Clone Number104
Concentration1.0 mg/ml
TargetCD45.2
ImmunogenB10.S mouse thymocytes and splenocytes
PurityThe antibody was purified by affinity chromatography.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C.
Regulatory StatusResearch Use Only

Scientific Background

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

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