Purified anti-mouse CD54 Antibody anti-CD54 - YN1/1.7.4,BioLegend,116101

Additional reported applications (for the relevant formats) include:in vitroandin vivoblocking of cell-cell adhesion and CD54 functions1,2,4, immunohistochemical staining3of acetone-fixed frozen sections, immunoprecipitation4, and Western blotting (non-reducing). The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 116131-116136).

Host

Rat

Reactivity

Mouse

Application

FC - Quality testedIHC-F - VerifiedIP, WB - Reported in the literature, not verified in house

Platform ID

BAB103328523

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NamePurified anti-mouse CD54 Antibody anti-CD54 - YN1/1.7.4
Cat. No.116101
HostRat
RRIDAB_313692 (BioLegend Cat. No. 116101)AB_313693 (BioLegend Cat. No. 116102)
IsotypeRat IgG2b, κ
ReactivityMouse
ApplicationFC - Quality testedIHC-F - VerifiedIP, WB - Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberYN1/1.7.4
Concentration0.5 mg/ml
TargetCD54
ImmunogenMouse NS-1 cells
PurityThe antibody was purified by affinity chromatography.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C.
Regulatory StatusResearch Use Only

Scientific Background

CD54 is a 90 kD immunoglobulin superfamily member also known as ICAM-1 and Ly-47. It is expressed on activated endothelial cells, high endothelial venules (HEV), T and B cells, monocytes/ macrophages, granulocytes, and dendritic cells. CD54 is an important intracellular adhesion molecule that participates in T cell-T cell, T cell-B cell, and T cell-target cell interactions via binding of LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). CD54 has also been shown to be involved in lymphocyte trafficking, making it an important molecule in many immune reactions and inflammation. CD54 is also a receptor for rhinovirus. The YN1/1.7.4 antibody has been reported to block binding of mouse CD54 to LFA-1 and Mac-1, inhibit cell-cell adhesion, and function in antigen presentation to T cells and leukocyte migration to inflammatory tissues.

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