Purified anti-mouse H-2Ld/H-2Db Antibody anti-H-2Ld, H-2Db - 28-14-8,BioLegend,114502
Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity1, immunoprecipitation3, blocking of cell lysis, and immunohistochemical staining of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 114510).Does not react with other haplotypes (e.g.,k, f, p, r, s).
Host
Mouse
Reactivity
Mouse
Application
FC -Quality testedIHC-F, IP -Reported in the literature, not verified in house
Platform ID
BAB486234755
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
Email:
Product Specifications
Scientific Background
Specifications
NamePurified anti-mouse H-2Ld/H-2Db Antibody anti-H-2Ld, H-2Db - 28-14-8
Cat. No.114502
HostMouse
RRIDAB_313583 (BioLegend Cat. No. 114502)
IsotypeMouse (C3H/HeJ) IgG2a, κ
ReactivityMouse
ApplicationFC -Quality testedIHC-F, IP -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone Number28-14-8
Concentration0.5 mg/ml
TargetH-2Ld/H-2Db
ImmunogenC3H.SW mouse splenocytes
PurityThe antibody was purified by affinity chromatography.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C.
Regulatory StatusResearch Use Only
Scientific Background
The 28-14-8 antibody reacts with the H-2DbMHC class I alloantigen expressed on nucleated cells from mice of the H-2Dbhaplotype. H-2Dbis involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 28-14-8 antibody has been reported to block antigen-specific lysis of target cells by cytotoxic T lymphocytes. The 28-14-8 antibody cross-reacts with the H-2Ldhaplotype. Clone 28-14-8 recognizes the α3 domain of H-2Db.
Category Paths
- Products>Primary Antibodies>IHC Antibodies
Request a product
Please provide the required information below so that we can quickly source your products.