ROCK1 (E2G4N) Rabbit Monoclonal Antibody#28999,Cell Signaling Technology (CST),28999

ROCK1 (E2G4N) Rabbit Monoclonal Antibody recognizes endogenous levels of total ROCK1 protein. Non-specific staining was observed in placenta by immunohistochemistry. In some cell lines, this antibody cross-reacts with 45-55 kDa bands of unknown origin by western blot.

Host

Rabbit

Reactivity

Human, Mouse, Rat

Application

Western Blotting: 1:1000 Simple WesternTM: 1:10 - 1:50 Immunohistochemistry (Paraffin): 1:400 - 1:1600 Immunofluorescence (Frozen): 1:400 - 1:1600 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600

Platform ID

BAB693012925

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NameROCK1 (E2G4N) Rabbit Monoclonal Antibody#28999
Cat. No.28999
Accession NumberQ13464
Gene ID (Entrez)13464, 6093
HostRabbit
SensitivityEndogenous
ReactivityHuman, Mouse, Rat
ApplicationWestern Blotting: 1:1000 Simple WesternTM: 1:10 - 1:50 Immunohistochemistry (Paraffin): 1:400 - 1:1600 Immunofluorescence (Frozen): 1:400 - 1:1600 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600
Molecular Weight160
ImmunogenIgG
FormulationSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C.Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #75673.
StorageSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C.Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #75673.
Regulatory StatusResearch Use Only

Scientific Background

ROCK (Rho-associated kinase), a family of serine/threonine kinases, is an important downstream target of Rho-GTPase and plays an important role in Rho-mediated signaling. Two isoforms of ROCK have been identified: ROCK1 and ROCK2. ROCK is composed of N-terminal catalytic, coiled-coil, and C-terminal PH (pleckstrin homology) domains. The C-terminus of ROCK negatively regulates its kinase activity (1,2). ROCK1 is cleaved by caspase-3 at a conserved DETD1113/G sequence resulting in loss of its C-terminal inhibitory domain (3). ROCK2 is directly cleaved by granzyme B (grB). Cleavage activates ROCK and leads to phosphorylation of myosin light chain (MLC) and inhibition of myosin phosphatase (4). This phosphorylation may account for the mechanism by which Rho regulates cytokinesis, cell motility, cell membrane blebbing during apoptosis, and smooth muscle contraction (5-7).Nakagawa, O. et al. (1996)FEBS Lett.392, 189-193.Lee, J.H. et al. (2004)J. Cell. Biol.167, 327-337.Sebbagh, M. et al. (2001)Nat Cell Biol3, 346-52.Sebbagh, M. et al. (2005)J Exp Med201, 465-71.Amano, M. et al. (1996)J Biol Chem271, 20246-9.Kureishi, Y. et al. (1997)J Biol Chem272, 12257-60.Totsukawa, G. et al. (2000)J Cell Biol150, 797-806.Alternate NamesMGC131603; MGC43611; p160 ROCK-1; p160-ROCK; p160ROCK; PRO0435; Renal carcinoma antigen NY-REN-35; Rho associated coiled-coil containing protein kinase 1; Rho kinase; Rho-associated protein kinase 1; Rho-associated, coiled-coil containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase I; ROCK-I; ROCK1

Synonyms

MGC131603; MGC43611; p160 ROCK-1; p160-ROCK; p160ROCK; PRO0435; Renal carcinoma antigen NY-REN-35; Rho associated coiled-coil containing protein kinase 1; Rho kinase; Rho-associated protein kinase 1; Rho-associated, coiled-coil containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase I; ROCK-I; ROCK1

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