Rab27B (E4V3O) Rabbit Monoclonal Antibody#17572,Cell Signaling Technology (CST),17572

Rab27B (E4V3O) Rabbit Monoclonal Antibody recognizes endogenous levels of total Rab27B protein. This antibody does not cross-react with Rab27A protein.

Host

Rabbit

Reactivity

Human

Application

Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:200 - 1:800 Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200

Platform ID

BAB691713549

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NameRab27B (E4V3O) Rabbit Monoclonal Antibody#17572
Cat. No.17572
Accession NumberO00194
Gene ID (Entrez)00194, 5874
HostRabbit
SensitivityEndogenous
ReactivityHuman
ApplicationWestern Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:200 - 1:800 Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200
Molecular Weight27
ImmunogenIgG
FormulationSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C.Do not aliquot the antibody.
StorageSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C.Do not aliquot the antibody.
Regulatory StatusResearch Use Only

Scientific Background

Rab27 proteins are members of the Ras superfamily of small Rab GTPases associated with the process of exocytosis (1-2), and in particular the exosome secretion pathway (3-5). There are two Rab27 isoforms, Rab27A and Rab27B, which are encoded by separate genes and which exhibit distinct subcellular localization and functions. Rab27A colocalizes to CD63+vesicles, whereas Rab27B is observed in perinuclear vesicles. Targeted knockdown studies suggest that the isoforms control distinct steps of the exosome secretion pathway (6). Rab27A participates in docking and membrane fusion from multivesicular endosomes (MVEs) to exosomes, whereas Rab27B mediates the transfer of membrane vesicles from the trans-Golgi network to MVEs. It has been suggested that Rab27A and Rab27B may have importance in exosome secretion by cancer cells (7).Fukuda, M. (2013)Traffic14, 949-63.Izumi, T. (2007)Endocr J54, 649-57.Elstak, E.D. et al. (2011)Blood118, 1570-8.Bahadoran, P. et al. (2001)J Cell Biol152, 843-50.Wood, S.M. et al. (2009)Blood114, 4117-27.Ostrowski, M. et al. (2010)Nat Cell Biol12, 19-30; sup pp 1-13.Li, Z. et al. (2018)Cell Commun Signal16, 44.Alternate NamesC25KG; RAB27B; RAB27B, member RAS oncogene family; Ras-related protein Rab-27B; RB27B

Synonyms

C25KG; RAB27B; RAB27B, member RAS oncogene family; Ras-related protein Rab-27B; RB27B

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