Rab8A (D22D8) Rabbit Monoclonal Antibody#6975,Cell Signaling Technology (CST),6975

Rab8A (D22D8) Rabbit Monoclonal Antibody recognizes endogenous levels of total Rab8A protein.

Host

Rabbit

Reactivity

Human, Mouse, Rat, Monkey

Application

Western Blotting: 1:1000 Immunoprecipitation: 1:200 Immunofluorescence (Immunocytochemistry): 1:200

Platform ID

BAB379307254

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NameRab8A (D22D8) Rabbit Monoclonal Antibody#6975
Cat. No.6975
Accession NumberP61006
Gene ID (Entrez)61006, 4218
HostRabbit
SensitivityEndogenous
ReactivityHuman, Mouse, Rat, Monkey
ApplicationWestern Blotting: 1:1000 Immunoprecipitation: 1:200 Immunofluorescence (Immunocytochemistry): 1:200
Molecular Weight23
ImmunogenIgG
FormulationSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
StorageSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Regulatory StatusResearch Use Only

Scientific Background

The Rab8 GTPase is a member of the Ras superfamily that functions in protein transport and membrane restructuring (1). Studies show that Rab8 is localized to the trans Golgi network (TGN), basolateral membrane, and vesicular structures where it helps regulate target protein transport between TGN and the basolateral membrane (1-3). Overexpression studies and mutation analysis of Rab8 and its associated Rab8GEF indicate additional roles in actin and microtubule remodeling during polarized membrane transport and membrane protrusion formation (4-6). Rab8 associates with myosin Vb and is required for translocation of GLUT4 following insulin stimulation in muscle (7,8). Control of target protein vesicle transport by Rab8 also regulates MT1-MMP activity during extracellular matrix formation and JRAB/MICAL-L2 at tight junction formation (9,10).Chen, Y.T. et al. (1993)Proc Natl Acad Sci USA90, 6508-12.Huber, L.A. et al. (1993)J Cell Biol123, 35-45.Henry, L. and Sheff, D.R. (2008)Mol Biol Cell19, 2059-68.Peränen, J. et al. (1996)J Cell Biol135, 153-67.Hattula, K. et al. (2002)Mol Biol Cell13, 3268-80.Hattula, K. et al. (2006)J Cell Sci119, 4866-77.Ishikura, S. and Klip, A. (2008)Am J Physiol Cell Physiol295, C1016-25.Randhawa, V.K. et al. (2008)J Biol Chem283, 27208-19.Bravo-Cordero, J.J. et al. (2007)EMBO J26, 1499-510.Yamamura, R. et al. (2008)Mol Biol Cell19, 971-83.Alternate NamesMEL; mel transforming oncogene (derived from cell line NK14); mel transforming oncogene (derived from cell line NK14)- RAB8 homolog; mel transforming oncogene (RAB8 homolog); Oncogene c-mel; RAB8; RAB8A; RAB8A, member RAS oncogene family; ras-associated protein RAB8; Ras-related protein Rab-8A

Synonyms

MEL; mel transforming oncogene (derived from cell line NK14); mel transforming oncogene (derived from cell line NK14)- RAB8 homolog; mel transforming oncogene (RAB8 homolog); Oncogene c-mel; RAB8; RAB8A; RAB8A, member RAS oncogene family; ras-associated protein RAB8; Ras-related protein Rab-8A

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